In order to verify the accuracy of the tritium production rate (TPR), neutron irradiation experiments have been performed with a mockup relevant to the fusion DEMO blanket consisting of F82H blocks, Li2TiO3 blocks with a 6Li enrichment of 40% and 95%, and beryllium blocks. Sample pellets of Li2TiO3 were irradiated and the TPR was measured by a liquid scintillation counter. The TPR was also calculated using the Monte Carlo code MCNP-4B with the nuclear data library JENDL-3.2 and ENDF-B/VI. The results agreed with experimental values within the statistical error (10%) of the experiment. Accordingly, it was clarified that the TPR could be evaluated within 10% uncertainty by the calculation code and the nuclear data. In order to estimate the induced activity caused by sequential reactions in cooling water pipes in the DEMO blanket, neutron irradiation experiments have been performed using test specimens simulating the pipes. Sample metals of Fe, W, Ti, Pb, Cu, V and reduced activation ferritic steel F82H were irradiated as typical fusion materials. The effective cross-sections needed to calculate the formation of the radioactive nuclei (56Co, 184Re, 48V, 206Bi, 65Zn and 51Cr) due to sequential reactions were measured. From the experimental results, it was found that the effective cross-sections increased remarkably while coming closer to polyethylene board, which was a substitute for water. As a result of this present study, it has become clear that the sequential reaction rates are important factors in the accurate evaluation of induced activity in fusion reactor design.