Species and impurity measurements have been made on the DITE Phase-II neutral-injection beams using both optical and charge-exchange techniques. The species measurements from the two techniques are in good agreement with one another. Hydrogen-bearing impurities originating from the sources have been detected and quantitative estimates of impurity content have been made. Strong evidence has been found for the impurity ions and being generated in the source at the few per cent level. There is evidence that the other principal impurity ion is H3O+ at a similar concentration. Measurements have also been made of spectral line emission from C3+ and O6+ in DITE plasmas with and without neutral injection in operation. Doppler-shifted spectra are observed, and their origin is discussed.