A systematic investigation of the effects of a parallel velocity shear and negative ions on the collisionless drift wave instability has for the first time been realized by simultaneously using a segmented tungsten hot plate of a Q-machine and sulfur hexafluoride (SF6) gas in a magnetized potassium plasma. The parallel velocity shear of the positive ion flow tends to decrease the fluctuation level of the drift wave. The introduction of negative ions first increases the fluctuation level and then starts to decrease it at the negative ion exchange fraction of around 10%, while keeping the above-mentioned shear effect qualitatively. In addition, a simple dispersion relation based on the local model has been calculated to show that it can predict wave characteristics similar to the experimental results. Our findings provide a potential for gaining a more profound insight into the physics of space/circumterrestrial plasmas.