Major disruptions, often caused by locked m/n = 2/1 modes (m/n is the poloidal/toroidal mode number), are great threat to a tokamak fusion reactor and should be mitigated or avoided. The locked 2/1 modes have been unlocked by electrode biasing (EB) for the first time in J-TEXT tokamak experiments. The application of a sufficiently negative EB voltage in plasma edge region quickly changes the phase and amplitude of the locked 2/1 mode and drives the mode to rotate in about 10–30 ms. For a larger EB current or a smaller locked mode amplitude, the mode is more easily to be unlocked, revealing a new method for mode unlocking and avoiding plasma disruptions.