Deuterium gas puff experiments were carried out on the S-300 Z-pinch at the Kurchatov Institute in Moscow. Gas puffs imploded onto the axis before a current peak at about 100 ns. Fusion neutrons were generated after the gas puff implosion during global expansion of a plasma column. Neutron emission lasted on average 35 ± 5 ns (full width half maximum, FWHM). In the downstream direction (on the Z-pinch axis behind the cathode), a mean neutron energy was 2.6 ± 0.1 MeV. Side-on neutron energy spectra peaked at 2.40 ± 0.05 MeV with about 600 ± 150 keV FWHM. A broad width of side-on neutron spectra implied a high radial component of deuteron velocities. An average kinetic energy of fast deuterons, which produced fusion neutrons, was 150 keV. A peak neutron yield reached a value of 6 × 1010 on a current level of 1.5 MA. It was by one order higher in comparison with other deuterated loads used on the same current generator. On the basis of experimental observations, we concluded that a total energy of deuterons accelerated to fusion energies was above 1.5 kJ. It is more than 15% of the energy input into a plasma. Therefore gas puff Z-pinches seem to be not only powerful sources of x-ray radiation but also efficient sources of 100 keV deuterons. Such a result is consistent with high neutron yields observed on the Angara Z-pinch and plasma foci with similar currents.